LAPTM4B antibody (Internal Region)

Catalog No. ABIN6262909

The Rabbit Polyclonal anti-LAPTM4B antibody (ABIN6262909) specifically detects LAPTM4B in WB, IHC, IF and ICC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for LAPTM4B antibody (Internal Region) (ABIN6262909)

Target: LAPTM4B (Lysosomal Protein Transmembrane 4 beta (LAPTM4B))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAPTM4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-LAPTM4B Antibody

Binding Specificity: Internal Region

Specificity: LAPTM4B Antibody detects endogenous levels of total LAPTM4B.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human LAPTM4B, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for LAPTM4B

Target: LAPTM4B (Lysosomal Protein Transmembrane 4 beta (LAPTM4B))

Alternative Name: LAPTM4B

Background:

Description: Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).

Gene: LAPTM4B

Molecular Weight: 24 kDa

Gene ID: 55353

UniProt: Q86VI4