Lactate Dehydrogenase A antibody (C-Term)

Catalog No. ABIN6262941

The Rabbit Polyclonal anti-Lactate Dehydrogenase A antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect Lactate Dehydrogenase A in samples from Human, Mouse and Rat. There are 4+ publications available.

Quick Overview for Lactate Dehydrogenase A antibody (C-Term) (ABIN6262941)

Target: Lactate Dehydrogenase A (LDHA)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lactate Dehydrogenase A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Lactate Dehydrogenase A Antibody

Binding Specificity: C-Term

Specificity: LDHA Antibody detects endogenous levels of total LDHA.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human LDHA, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-Lactate Dehydrogenase A antibody (ABIN6262941)

Zhang, Wang, Han: "MicroRNA‑34a inhibits liver cancer cell growth by reprogramming glucose metabolism." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 4483-4489, (2018) (PubMed).

Zhang, Song, Sun, Li, Chen, Yang, Xing: "AMPK/GSK3β/β-catenin cascade-triggered overexpression of CEMIP promotes migration and invasion in anoikis-resistant prostate cancer cells by enhancing metabolic reprogramming." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 7, pp. 3924-3935, (2018) (PubMed).

Huang, Li, Xie, Ye, Chen, Song, Tang, Xie: "High expressions of LDHA and AMPK as prognostic biomarkers for breast cancer." in: Breast (Edinburgh, Scotland), Vol. 30, pp. 39-46, (2017) (PubMed).

Xiao, Huang, Ye, Chen, Song, Wen, Zhang, Zheng, Tang, Xie: "The miR-34a-LDHA axis regulates glucose metabolism and tumor growth in breast cancer." in: Scientific reports, Vol. 6, pp. 21735, (2016) (PubMed).

Target Details for Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: LDHA

Background:

Description: (S)-lactate + NAD+ = pyruvate + NADH.

Gene: LDHA

Molecular Weight: 37kDa

Gene ID: 3939

UniProt: P00338

Pathways: Warburg Effect