MAGEA2B antibody (C-Term)

Catalog No. ABIN6263054

This Rabbit Polyclonal antibody specifically detects MAGEA2B in ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for MAGEA2B antibody (C-Term) (ABIN6263054)

Target: MAGEA2B (Melanoma Antigen Family A, 2B (MAGEA2B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAGEA2B antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-MAGEA2B Antibody

Binding Specificity: C-Term

Specificity: MAGEA2 Antibody detects endogenous levels of total MAGEA2.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MAGEA2, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MAGEA2B

Target: MAGEA2B (Melanoma Antigen Family A, 2B (MAGEA2B))

Alternative Name: MAGEA2, MAGEA2B

Background:

Description: Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. Negatively regulates acetylation and sumoylation of PML and represses PML-induced p53/TP53 acetylation and activation.

Gene: MAGEA2, MAGEA2B

Molecular Weight: 35 kDa

Gene ID: 266740, 4101

UniProt: P43356