MAGEF1 antibody (Internal Region)

Catalog No. ABIN6263078

This Rabbit Polyclonal antibody specifically detects MAGEF1 in WB, IHC, ICC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for MAGEF1 antibody (Internal Region) (ABIN6263078)

Target: MAGEF1 (Melanoma Antigen Family F, 1 (MAGEF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAGEF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-MAGEF1 Antibody

Binding Specificity: Internal Region

Purpose: MAGF1 Antibody

Specificity: MAGF1 Antibody detects endogenous levels of total MAGF1.

Predicted Reactivity: Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MAGF1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: IF/ICC 1:100-1:500,WB 1:1000-3000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MAGEF1

Target: MAGEF1 (Melanoma Antigen Family F, 1 (MAGEF1))

Alternative Name: MAGF1

Background:

Description: Enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin ligases. Proposed to act through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. MAGEF1-NSMCE1 ubiquitin ligase complex promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. May negatively regulate genome integrity by inhibiting homologous recombination-mediated double-strand break DNA repair (PubMed:29225034).

Gene: MAGEF1

Molecular Weight: 35 kDa

Gene ID: 64110

UniProt: Q9HAY2