MBD2 antibody (Internal Region)

Catalog No. ABIN6263148

The Rabbit Polyclonal anti-MBD2 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect MBD2 in samples from Human and Mouse.

Quick Overview for MBD2 antibody (Internal Region) (ABIN6263148)

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MBD2 Antibody

Binding Specificity: Internal Region

Specificity: MBD2 Antibody detects endogenous levels of total MBD2.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MBD2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MBD2

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Alternative Name: MBD2

Background:

Description: Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.

Gene: MBD2

Molecular Weight: 43 kDa

Gene ID: 8932

UniProt: Q9UBB5

Pathways: Chromatin Binding