MBNL1 antibody (C-Term)

Catalog No. ABIN6263151

The Rabbit Polyclonal anti-MBNL1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect MBNL1 in samples from Human.

Quick Overview for MBNL1 antibody (C-Term) (ABIN6263151)

Target: MBNL1 (Muscleblind-like Protein 1 (MBNL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBNL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-MBNL1 Antibody

Binding Specificity: C-Term

Specificity: MBNL1 Antibody detects endogenous levels of total MBNL1.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MBNL1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MBNL1

Target: MBNL1 (Muscleblind-like Protein 1 (MBNL1))

Alternative Name: MBNL1

Background:

Description: Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

Gene: MBNL1

Molecular Weight: 42 kDa

Gene ID: 4154

UniProt: Q9NR56

Pathways: Ribonucleoprotein Complex Subunit Organization