MECP2 antibody (N-Term)
Quick Overview for MECP2 antibody (N-Term) (ABIN6263185)
Target
See all MECP2 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- N-Term
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Specificity
- MeCP2 Antibody detects endogenous levels of total MeCP2.
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Predicted Reactivity
- Horse,Rabbit,Dog
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Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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Immunogen
- A synthesized peptide derived from human MeCP2, corresponding to a region within N-terminal amino acids.
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Isotype
- IgG
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Application Notes
- WB 1:1000-2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
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Expiry Date
- 12 months
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- MECP2 (Methyl CpG Binding Protein 2 (MECP2))
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Alternative Name
- MECP2
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Background
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Description: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Gene: MECP2
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Molecular Weight
- 53 kDa.
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Gene ID
- 4204
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UniProt
- P51608
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Pathways
- Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane
Target
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