MTAP antibody (Internal Region)

Catalog No. ABIN6263418

The Rabbit Polyclonal anti-MTAP antibody is suitable to detect MTAP in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ICC.

Quick Overview for MTAP antibody (Internal Region) (ABIN6263418)

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MTAP Antibody

Binding Specificity: Internal Region

Purpose: MTAP Antibody

Specificity: MTAP Antibody detects endogenous levels of total MTAP.

Predicted Reactivity: Zebrafish,Bovine,Sheep,Rabbit,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MTAP, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MTAP

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Alternative Name: MTAP

Background:

Description: Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.

Gene: MTAP

Molecular Weight: 31kDa

Gene ID: 4507

UniProt: Q13126

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process