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NEFL antibody (N-Term)

The Rabbit Polyclonal anti-NEFL antibody has been validated for WB, IHC, IF, ELISA and ICC. It is suitable to detect NEFL in samples from Human, Rat and Mouse.
Catalog No. ABIN6263586

Quick Overview for NEFL antibody (N-Term) (ABIN6263586)

Target

See all NEFL Antibodies
NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity

  • 111
  • 51
  • 44
  • 27
  • 24
  • 18
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Rat, Mouse

Host

  • 89
  • 48
  • 8
  • 1
  • 1
  • 1
Rabbit

Clonality

  • 76
  • 72
Polyclonal

Conjugate

  • 99
  • 19
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This NEFL antibody is un-conjugated

Application

  • 89
  • 71
  • 41
  • 33
  • 33
  • 32
  • 26
  • 16
  • 14
  • 13
  • 11
  • 10
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)
  • Binding Specificity

    • 15
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Specificity

    NEFL Antibody detects endogenous levels of total NEFL.

    Predicted Reactivity

    Chicken

    Purification

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    Immunogen

    A synthesized peptide derived from human NEFL, corresponding to a region within N-terminal amino acids.

    Isotype

    IgG
  • Application Notes

    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20 °C. Stable for 12 months from date of receipt.

    Expiry Date

    12 months
  • Target

    NEFL (Neurofilament, Light Polypeptide (NEFL))

    Alternative Name

    NEFL

    Background

    Description: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

    Gene: NEFL

    Molecular Weight

    62kDa

    Gene ID

    4747

    UniProt

    P07196
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