NEK2 antibody (Internal Region)

Catalog No. ABIN6263590

The Rabbit Polyclonal anti-NEK2 antibody (ABIN6263590) specifically detects NEK2 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NEK2 antibody (Internal Region) (ABIN6263590)

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NEK2 Antibody

Binding Specificity: Internal Region

Specificity: NEK2 Antibody detects endogenous levels of total NEK2.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NEK2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Alternative Name: NEK2

Background:

Description: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC, SGO1, NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856).

Gene: NEK2

Molecular Weight: 51kDa

Gene ID: 4751

UniProt: P51955

Pathways: M Phase