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Nestin antibody (C-Term)

The Rabbit Polyclonal anti-Nestin antibody (ABIN6263599) specifically detects Nestin in IHC, WB, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

IF/ICC

2 images

Catalog No. ABIN6263599

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Quick Overview for Nestin antibody (C-Term) (ABIN6263599)

Target

See all Nestin (NES) Antibodies

Nestin (NES)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Nestin antibody is un-conjugated

Application

Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product NES Primary Antibody

Binding Specificity
16

16

11

7

6

5

5

5

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
C-Term

Purpose

Nestin Antibody

Specificity

Nestin Antibody detects endogenous levels of total Nestin.

Predicted Reactivity

Rabbit,Dog

Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen

A synthesized peptide derived from human Nestin, corresponding to a region within C-terminal amino acids.

Isotype

IgG
anti-Nestin (NES) (AA 1389-1416) antibody
NES Reactivity: Human WB, FACS, IF, IHC (p) Host: Rabbit Polyclonal RB16253 unconjugated

anti-Nestin (NES) (AA 178-399) antibody
NES Reactivity: Human IHC, WB, FACS Host: Mouse Monoclonal D10 unconjugated

anti-Nestin (NES) antibody
NES Reactivity: Human IHC, WB, FACS, IF, ELISA Host: Mouse Monoclonal 2C2F7 unconjugated

anti-Nestin (NES) antibody
NES Reactivity: Mouse IHC, ICC Host: Rat Monoclonal 7A3 unconjugated

anti-Nestin (NES) (N-Term) antibody
NES Reactivity: Human, Mouse, Rat IHC, WB Host: Rabbit Polyclonal unconjugated

anti-Nestin (NES) (AA 600-900) antibody
NES Reactivity: Human IHC Host: Mouse Monoclonal NES-2911 unconjugated

anti-Nestin (NES) (AA 1464-1614) antibody (FITC)
NES Reactivity: Human FACS, IF, ICC Host: Mouse Monoclonal 10C2 FITC

anti-Nestin (NES) (AA 1484-1500) antibody
NES Reactivity: Human, Mouse IHC, WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Nestin (NES) (AA 1389-1416) antibody
NES Reactivity: Human IHC, WB, FACS, IF, ELISA Host: Rabbit Polyclonal unconjugated

anti-Nestin (NES) (AA 419-588) antibody
NES Reactivity: Human WB, FACS, ELISA Host: Mouse Monoclonal 1A9G12 unconjugated

Application Notes

WB 1:1000-3000, IHC 1:50-200, IF/ICC 1:100-1:500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date

12 months
Target

Nestin (NES)

Alternative Name

Nestin

Background

Description: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Gene: NES

Molecular Weight

200 kDa

Gene ID

10763

UniProt

P48681