ORC4 antibody (C-Term)

Catalog No. ABIN6263904

The Rabbit Polyclonal anti-ORC4 antibody is suitable to detect ORC4 in samples from Human, Mouse and Rat. It has been validated for ELISA and WB.

Quick Overview for ORC4 antibody (C-Term) (ABIN6263904)

Target: ORC4 (Origin Recognition Complex, Subunit 4 (ORC4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORC4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-ORC4 Antibody

Binding Specificity: C-Term

Specificity: ORC4L Antibody detects endogenous levels of total ORC4L.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ORC4L, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ORC4

Target: ORC4 (Origin Recognition Complex, Subunit 4 (ORC4))

Alternative Name: ORC4

Background:

Description: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.

Gene: ORC4

Molecular Weight: 50 kDa

Gene ID: 5000

UniProt: O43929

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA