PASK antibody (C-Term)

Catalog No. ABIN6264045

The Rabbit Polyclonal anti-PASK antibody has been validated for WB and ELISA. It is suitable to detect PASK in samples from Human and Mouse.

Quick Overview for PASK antibody (C-Term) (ABIN6264045)

Target: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PASK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PASK Antibody

Binding Specificity: C-Term

Specificity: PASK Antibody detects endogenous levels of total PASK.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PASK, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PASK

Target: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Alternative Name: PASK

Background:

Description: Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells, however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation.

Gene: PASK

Molecular Weight: 142kDa

Gene ID: 23178

UniProt: Q96RG2

Pathways: Regulation of Carbohydrate Metabolic Process