PI3K p85/p55 antibody (Internal Region)

Catalog No. ABIN6264193

Cited in 1 publication. The Rabbit Polyclonal anti-PI3K p85/p55 antibody (ABIN6264193) specifically detects PI3K p85/p55 in IHC, WB, IF and ICC. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for PI3K p85/p55 antibody (Internal Region) (ABIN6264193)

Target: PI3K p85/p55 (Phosphoinositide 3 Kinase, p85/p55 (PI3K p85/p55))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI3K p85/p55 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PI3K p85/p55 Antibody

Binding Specificity: Internal Region

Purpose: PI3K p85/p55 Antibody

Specificity: PI3-kinase p85-alpha/gamma Antibody detects endogenous levels of total PI3-kinase p85-alpha/gamma

Predicted Reactivity: Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PI3K p85p55, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-PI3K p85/p55 antibody (ABIN6264193)

Chai, Bai, Li, Chen, Zhang: "Biological functions of lung cancer cells are suppressed in co-culture with mesenchymal stem cells isolated from umbilical cord." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1076-1080, (2018) (PubMed).

Target Details for PI3K p85/p55

Target: PI3K p85/p55 (Phosphoinositide 3 Kinase, p85/p55 (PI3K p85/p55))

Alternative Name: PI3K p85/p55

Background:

Description: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

Gene: PIK3R1

Molecular Weight: 54,83kDa

Gene ID: 5295

UniProt: P27986