PIEZO1 antibody (Internal Region)

Catalog No. ABIN6264200

This Rabbit Polyclonal antibody specifically detects PIEZO1 in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for PIEZO1 antibody (Internal Region) (ABIN6264200)

Target: PIEZO1 (Piezo-Type Mechanosensitive Ion Channel Component 1 (PIEZO1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIEZO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PIEZO1 Antibody

Binding Specificity: Internal Region

Specificity: Piezo1 Antibody detects endogenous levels of total Piezo1.

Predicted Reactivity: Pig,Bovine,Horse,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Piezo1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PIEZO1

Target: PIEZO1 (Piezo-Type Mechanosensitive Ion Channel Component 1 (PIEZO1))

Alternative Name: PIEZO1

Background:

Description: Pore-forming subunit of a mechanosensitive non-specific cation channel (PubMed:23479567, PubMed:23695678). Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling (PubMed:20016066). In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation (PubMed:25119035). Plays a key role in blood vessel formation and vascular structure in both development and adult physiology (By similarity).

Gene: PIEZO1

Molecular Weight: 287 kDa

Gene ID: 9780

UniProt: Q92508