POLR3B antibody (C-Term)

Catalog No. ABIN6264349

This anti-POLR3B antibody is a Rabbit Polyclonal antibody detecting POLR3B in WB, ELISA, IF and ICC. Suitable for Human and Rat.

Quick Overview for POLR3B antibody (C-Term) (ABIN6264349)

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLR3B Antibody

Binding Specificity: C-Term

Specificity: POLR3B Antibody detects endogenous levels of total POLR3B.

Predicted Reactivity: Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POLR3B, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for POLR3B

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Alternative Name: POLR3B

Background:

Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Gene: POLR3B

Molecular Weight: 128kDa

Gene ID: 55703

UniProt: Q9NW08