POLR3E antibody (N-Term)

Catalog No. ABIN6264350

This Rabbit Polyclonal antibody specifically detects POLR3E in ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for POLR3E antibody (N-Term) (ABIN6264350)

Target: POLR3E (Polymerase (RNA) III (DNA Directed) Polypeptide E (80kD) (POLR3E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3E antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-POLR3E Antibody

Binding Specificity: N-Term

Specificity: POLR3E Antibody detects endogenous levels of total POLR3E.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POLR3E, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for POLR3E

Target: POLR3E (Polymerase (RNA) III (DNA Directed) Polypeptide E (80kD) (POLR3E))

Alternative Name: POLR3E

Background:

Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).

Gene: POLR3E

Molecular Weight: 80kDa

Gene ID: 55718

UniProt: Q9NVU0