PRPF8 antibody (C-Term)

Catalog No. ABIN6264449

This Rabbit Polyclonal antibody specifically detects PRPF8 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRPF8 antibody (C-Term) (ABIN6264449)

Target: PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRPF8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PRPF8 Antibody

Binding Specificity: C-Term

Specificity: PRPF8 Antibody detects endogenous levels of total PRPF8.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PRPF8, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PRPF8

Target: PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))

Alternative Name: PRPF8

Background:

Description: Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

Gene: PRPF8

Molecular Weight: 220 kDa

Gene ID: 10594

UniProt: Q6P2Q9