PTTG1 antibody (Internal Region)

Catalog No. ABIN6264528

The Rabbit Polyclonal anti-PTTG1 antibody has been validated for ELISA, WB, IHC, IF and ICC. It is suitable to detect PTTG1 in samples from Human.

Quick Overview for PTTG1 antibody (Internal Region) (ABIN6264528)

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTTG1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PTTG1 Antibody

Binding Specificity: Internal Region

Specificity: PTTG1 Antibody detects endogenous levels of total PTTG1.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PTTG1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IF/ICC: 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PTTG1

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Alternative Name: PTTG1

Background:

Description: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.

Gene: PTTG1

Molecular Weight: 30kDa

Gene ID: 9232

UniProt: O95997