TP53BP1 antibody (Tumor Protein P53 Binding Protein 1)

Details for Product anti-TP53BP1 Antibody No. ABIN6264615
Target
Reactivity
Human, Mouse, Rat
131
78
54
11
4
4
4
3
2
2
1
1
1
Host
Rabbit
118
11
2
1
Clonality
Polyclonal
Conjugate
This TP53BP1 antibody is un-conjugated
9
9
6
6
6
6
6
2
2
2
2
2
2
2
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
70
40
39
28
27
21
17
14
8
5
3
3
1
1
1
Options
Immunogen A synthesized peptide derived from human RAD51
Isotype IgG
Specificity TP53BP1 Antibody detects endogenous levels of total TP53BP1
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Alternative Name TP53BP1 (TP53BP1 Antibody Abstract)
Background

Description: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).

Gene: TP53BP1

Molecular Weight 213kDa
Gene ID 7158
UniProt Q12888
Pathways DNA Damage Repair
Application Notes WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months
Images
Image no. 1 for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody (ABIN6264615) ABIN6277712 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde...
Image no. 2 for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody (ABIN6264615) Western blot analysis of extracts of A431 , using TP53BP1 antibody. The lane on the l...
Image no. 3 for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody (ABIN6264615) ABIN6277712 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...