TP53BP1 antibody (C-Term)

Catalog No. ABIN3044038

This Rabbit Polyclonal antibody specifically detects TP53BP1 in WB, IHC and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TP53BP1 antibody (C-Term) (ABIN3044038)

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TP53BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-TP53BP1 Antibody

Binding Specificity: AA 1955-1972, C-Term

Purpose: Anti-53BP1/TP53BP1 Antibody

Sequence: ERIGFKQHPK YKHDYVSH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-53BP1/TP53BP1 Antibody (ABIN3044038). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TP53BP1

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Alternative Name: TP53BP1

Background:

Synonyms: Tumor suppressor p53-binding protein 1,53BP1,p53-binding protein 1,p53BP1,TP53BP1,

Tissue Specificity: Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells.

Background: TP53BP1 (Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al. (1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al. (1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al. (1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21 (CDKN1A). Wang et al. (2002) used small interfering RNA ed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.

Sequence Similarities: Contains 2 BRCT domains.

Molecular Weight: 450 kDa

UniProt: Q12888

Pathways: DNA Damage Repair