Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 1955-1972) Primary Antibody

TP53BP1 Reactivity: Human, Mouse, Rat ICC, IHC (p), WB Host: Rabbit Polyclonal
Catalog No. ABIN3044038
$280.00
Plus shipping costs $45.00
100 μg
local_shipping Shipping to: United States
Delivery in 4 to 6 Business Days
  • Target
    Binding Specificity
    AA 1955-1972, C-Term
    Reactivity
    Human, Mouse, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This TP53BP1 antibody is un-conjugated
    Application
    Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Tumor suppressor p53-binding protein 1(TP53BP1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
    Sequence
    ERIGFKQHPK YKHDYVSH
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Tumor suppressor p53-binding protein 1(TP53BP1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
    Gene Name: tumor protein p53 binding protein 1
    Protein Name: Tumor suppressor p53-binding protein 1
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence.
    Isotype
    IgG
  • Target
    Alternative Name
    TP53BP1 (TP53BP1 Antibody Abstract)
    Background
    TP53BP1(Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al.(1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al.(1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al.(1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21(CDKN1A). Wang et al.(2002) used small interfering RNA directed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.

    Synonyms: 53 BP1 antibody|53BP1 antibody|FLJ41424 antibody|MGC138366 antibody|p202 antibody|p53 binding protein 1 antibody|p53 BP1 antibody|p53-binding protein 1 antibody|p53BP1 antibody|TP53 BP1 antibody|TP53B_HUMAN antibody|TP53BP1 antibody|TRP53 BP1 antibody|Tumor protein 53 binding protein 1 antibody|Tumor protein p53 binding protein 1 antibody|Tumor suppressor p53 binding protein 1 antibody|Tumor suppressor p53-binding protein 1 antibody
    UniProt
    Q12888
    Pathways
    DNA Damage Repair
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat, The detection limit for TP53BP1 is approximately 2.5 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
You are here:
help Support