RNF5 antibody (C-Term)

Catalog No. ABIN6264790

This anti-RNF5 antibody is a Rabbit Polyclonal antibody detecting RNF5 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for RNF5 antibody (C-Term) (ABIN6264790)

Target: RNF5 (Ring Finger Protein 5, E3 Ubiquitin Protein Ligase (RNF5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RNF5 Antibody

Binding Specificity: C-Term

Specificity: RNF5 Antibody detects endogenous levels of total RNF5.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RNF5, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RNF5

Target: RNF5 (Ring Finger Protein 5, E3 Ubiquitin Protein Ligase (RNF5))

Alternative Name: RNF5

Background:

Description: Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD, the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation, the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses.

Gene: RNF5

Molecular Weight: 20 kDa

Gene ID: 6048

UniProt: Q99942