Replication Protein A 30 KDa Subunit (RPA30) (Internal Region) antibody

Catalog No. ABIN6264810

The Rabbit Polyclonal anti-Replication Protein A 30 KDa Subunit antibody is suitable to detect Replication Protein A 30 KDa Subunit in samples from Human and Mouse. It has been validated for ICC, IF and WB.

Quick Overview for Replication Protein A 30 KDa Subunit (RPA30) (Internal Region) antibody (ABIN6264810)

Target: Replication Protein A 30 KDa Subunit (RPA30)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Product Details

Binding Specificity: Internal Region

Purpose: RPA30 Antibody

Specificity: RPA30 Antibody detects endogenous levels of total RPA30.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RPA30, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: Replication Protein A 30 KDa Subunit (RPA30)

Alternative Name: RPA30

Background:

Description: As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Gene: RPA4

Molecular Weight: 28 kDa

Gene ID: 29935

UniProt: Q13156

Pathways: Mitotic G1-G1/S Phases, DNA Replication