RUNX3 antibody (Internal Region)

Catalog No. ABIN6264885

The Rabbit Polyclonal anti-RUNX3 antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect RUNX3 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for RUNX3 antibody (Internal Region) (ABIN6264885)

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RUNX3 Antibody

Binding Specificity: Internal Region

Specificity: RUNX3 Antibody detects endogenous levels of total RUNX3.

Predicted Reactivity: Pig,Bovine,Horse,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RUNX3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-RUNX3 antibody (ABIN6264885)

Li, Zhao, Huang, Wang, Zhu, Cao, Xiong, Deng: "MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway." in: Gene, Vol. 641, pp. 240-247, (2017) (PubMed).

Target Details for RUNX3

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Alternative Name: RUNX3

Background:

Description: Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).

Gene: RUNX3

Molecular Weight: 44 kDa

Gene ID: 864

UniProt: Q13761