RUNX3 antibody (Internal Region)

Catalog No. ABIN6264885

There is 1 publication for this product available. The Rabbit Polyclonal anti-RUNX3 antibody is suitable to detect RUNX3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ICC.

Quick Overview for RUNX3 antibody (Internal Region) (ABIN6264885)

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RUNX3 Antibody

Binding Specificity: Internal Region

Purpose: RUNX3 Antibody

Specificity: RUNX3 Antibody detects endogenous levels of total RUNX3.

Predicted Reactivity: Pig,Bovine,Horse,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RUNX3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-RUNX3 antibody (ABIN6264885)

Li, Zhao, Huang, Wang, Zhu, Cao, Xiong, Deng: "MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway." in: Gene, Vol. 641, pp. 240-247, (2017) (PubMed).

Target Details for RUNX3

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Alternative Name: RUNX3

Background:

Description: Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).

Gene: RUNX3

Molecular Weight: 44 kDa

Gene ID: 864

UniProt: Q13761