TCF4 antibody (N-Term)

Catalog No. ABIN6265500

The Rabbit Polyclonal anti-TCF4 antibody has been validated for WB, ELISA and IHC. It is suitable to detect TCF4 in samples from Human, Mouse and Rat.

Quick Overview for TCF4 antibody (N-Term) (ABIN6265500)

Target: TCF4 (Transcription Factor 4 (TCF4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TCF4 Antibody

Binding Specificity: N-Term

Specificity: TCF4 Antibody detects endogenous levels of total TCF4.

Predicted Reactivity: Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TCF4, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TCF4

Target: TCF4 (Transcription Factor 4 (TCF4))

Alternative Name: TCF4

Background:

Description: Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.

Gene: TCF4

Molecular Weight: 71kDa

Gene ID: 6925

UniProt: P15884

Pathways: WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus