PAR1 antibody (N-Term)

Catalog No. ABIN6265563

The Rabbit Polyclonal anti-PAR1 antibody has been validated for WB, ELISA, IHC, ICC and IF. It is suitable to detect PAR1 in samples from Human, Rat and Mouse. There are 2+ publications available.

Quick Overview for PAR1 antibody (N-Term) (ABIN6265563)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PAR1 Antibody

Binding Specificity: N-Term

Specificity: Thrombin Receptor Antibody detects endogenous levels of total Thrombin Receptor.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Thrombin Receptor, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-PAR1 antibody (ABIN6265563)

Zhao, Zheng, Liu, Luo, Wu, Liu, Zou: "Inhibiting trophoblast PAR-1 overexpression suppresses sFlt-1-induced anti-angiogenesis and abnormal vascular remodeling: a possible therapeutic approach for preeclampsia." in: Molecular human reproduction, Vol. 24, Issue 3, pp. 158-169, (2018) (PubMed).

Zhong, Chen, Zhang, Xiao, Qin, Gu, Sun, Liu, Jing, Hu, Zhang, Zhou, Sun, Yang: "Doxycycline directly targets PAR1 to suppress tumor progression." in: Oncotarget, Vol. 8, Issue 10, pp. 16829-16842, (2017) (PubMed).

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: F2R

Background:

Description: High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

Gene: F2R

Molecular Weight: 48kDa

Gene ID: 2149

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus