PAR1 antibody (N-Term)

Catalog No. ABIN6265563

There are 2+ publications for this product available. The Rabbit Polyclonal anti-PAR1 antibody is suitable to detect PAR1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and ICC.

Quick Overview for PAR1 antibody (N-Term) (ABIN6265563)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PAR1 Antibody

Binding Specificity: N-Term

Specificity: Thrombin Receptor Antibody detects endogenous levels of total Thrombin Receptor.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Thrombin Receptor, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-PAR1 antibody (ABIN6265563)

Zhao, Zheng, Liu, Luo, Wu, Liu, Zou: "Inhibiting trophoblast PAR-1 overexpression suppresses sFlt-1-induced anti-angiogenesis and abnormal vascular remodeling: a possible therapeutic approach for preeclampsia." in: Molecular human reproduction, Vol. 24, Issue 3, pp. 158-169, (2018) (PubMed).

Zhong, Chen, Zhang, Xiao, Qin, Gu, Sun, Liu, Jing, Hu, Zhang, Zhou, Sun, Yang: "Doxycycline directly targets PAR1 to suppress tumor progression." in: Oncotarget, Vol. 8, Issue 10, pp. 16829-16842, (2017) (PubMed).

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: F2R

Background:

Description: High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

Gene: F2R

Molecular Weight: 48kDa

Gene ID: 2149

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus