TOPBP1 antibody (Internal Region)

Catalog No. ABIN6265675

This anti-TOPBP1 antibody is a Rabbit Polyclonal antibody detecting TOPBP1 in WB and ELISA. Suitable for Human and Mouse.

Quick Overview for TOPBP1 antibody (Internal Region) (ABIN6265675)

Target: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOPBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TOPBP1 Antibody

Binding Specificity: Internal Region

Specificity: TOPBP1 Antibody detects endogenous levels of total TOPBP1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TOPBP1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TOPBP1

Target: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Alternative Name: TOPBP1

Background:

Description: Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR (PubMed:16530042).

Gene: TOPBP1

Molecular Weight: 173kDa

Gene ID: 11073

UniProt: Q92547

Pathways: Chromatin Binding