UBE2L3 antibody (C-Term)

Catalog No. ABIN6265850

This Rabbit Polyclonal antibody specifically detects UBE2L3 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for UBE2L3 antibody (C-Term) (ABIN6265850)

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2L3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-UBE2L3 Antibody

Binding Specificity: C-Term

Specificity: UBE2L3 Antibody detects endogenous levels of total UBE2L3.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human UBE2L3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for UBE2L3

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: UBE2L3

Background:

Description: Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.

Gene: UBE2L3

Molecular Weight: 18 kDa

Gene ID: 7332

UniProt: P68036