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Vinculin antibody (N-Term)

The Rabbit Polyclonal anti-Vinculin antibody is suitable to detect Vinculin in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Western blot analysis of vinculin expression in HepG2,The lane on the left is treated with the antigen-specific peptide. (Vinculin antibody (N-Term))

ABIN6268668 at 1/100 staining Mouse muscle tissue by IHC-P. (Vinculin antibody (N-Term))

IHC

2 images

Catalog No. ABIN6265986

$494.77

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Quick Overview for Vinculin antibody (N-Term) (ABIN6265986)

Target

See all Vinculin (VCL) Antibodies

Vinculin (VCL)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Vinculin antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Discover our top product VCL Primary Antibody

Binding Specificity
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N-Term

Specificity

Vinculin Antibody detects endogenous levels of total Vinculin.

Predicted Reactivity

Pig,Bovine,Horse,Rabbit,Dog,Chicken

Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen

A synthesized peptide derived from human Vinculin, corresponding to a region within N-terminal amino acids.

Isotype

IgG
anti-Vinculin (VCL) (AA 1-1066) antibody
VCL Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 2C6-1B5 unconjugated

anti-Vinculin (VCL) (AA 879-1064) antibody
VCL Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Vinculin (VCL) (AA 903-937) antibody
VCL Reactivity: Human, Mouse, Rat WB, IHC (p), FACS Host: Rabbit Polyclonal RB55983 unconjugated

anti-Vinculin (VCL) (AA 1020-1134) antibody
VCL Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Vinculin (VCL) (AA 767-1066) antibody
VCL Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vinculin (VCL) (AA 634-668) antibody
VCL Reactivity: Human, Mouse, Rat WB, IHC (p), FACS Host: Rabbit Polyclonal RB55982 unconjugated

anti-Vinculin (VCL) (AA 12-39), (N-Term) antibody
VCL Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal RB15469 unconjugated

anti-Vinculin (VCL) (AA 1-1066) antibody
VCL Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3F8-1D4 unconjugated

anti-Vinculin (VCL) (AA 174-322) antibody
VCL Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal VCL-2572 unconjugated

anti-Vinculin (VCL) (AA 174-322) antibody
VCL Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal VCL-2575 unconjugated

Application Notes

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date

12 months
Target

Vinculin (VCL)

Alternative Name

VCL

Background

Description: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Gene: VCL

Molecular Weight

130 kDa

Gene ID

7414

UniProt

P18206

Pathways

Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2