XRCC3 antibody (N-Term)

Catalog No. ABIN6266068

This anti-XRCC3 antibody is a Rabbit Polyclonal antibody detecting XRCC3 in WB, ELISA, IHC, IF and ICC. Suitable for Human and Rat.

Quick Overview for XRCC3 antibody (N-Term) (ABIN6266068)

Target: XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-XRCC3 Antibody

Binding Specificity: N-Term

Specificity: XRCC3 Antibody detects endogenous levels of total XRCC3.

Predicted Reactivity: Horse,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human XRCC3, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for XRCC3

Target: XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3))

Alternative Name: XRCC3

Background:

Description: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD21 paralog protein complex CX3 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment, the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process, the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.

Gene: XRCC3

Molecular Weight: 38kDa

Gene ID: 7517

UniProt: O43542

Pathways: DNA Damage Repair