Histone Cluster 2, H4a (HIST2H4A) (Lys20) antibody

Catalog No. ABIN6266164

The Rabbit Polyclonal anti-Histone Cluster 2, H4a antibody (ABIN6266164) specifically detects Histone Cluster 2, H4a in WB, ELISA, ICC, ChIP, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Histone Cluster 2, H4a (HIST2H4A) (Lys20) antibody (ABIN6266164)

Target: Histone Cluster 2, H4a (HIST2H4A)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details

Binding Specificity: Lys20

Specificity: HIST2H4A Antibody detects endogenous levels of total HIST2H4A

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IF 1:50-1:200 IP 1:50-1:200 CHIP 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details

Target: Histone Cluster 2, H4a (HIST2H4A)

Alternative Name: HIST2H4A

Background:

Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene: HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4

Molecular Weight: 11kDa

Gene ID: 121, 504, 554

UniProt: P62805

Pathways: Telomere Maintenance