TCF7 antibody (N-Term)

Catalog No. ABIN6266277

The Rabbit Polyclonal anti-TCF7 antibody is suitable to detect TCF7 in samples from Human, Mouse and Rat. It has been validated for WB, IF and ICC.

Quick Overview for TCF7 antibody (N-Term) (ABIN6266277)

Target: TCF7 (Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-TCF7 Antibody

Binding Specificity: N-Term

Purpose: TCF7 Antibody

Specificity: TCF7 Antibody detects endogenous levels of total TCF7

Predicted Reactivity: Pig,Bovine,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TCF7, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TCF7

Target: TCF7 (Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7))

Alternative Name: TCF7

Background:

Description: Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.

Gene: TCF7

Molecular Weight: 42kDa

Gene ID: 6932

UniProt: P36402

Pathways: WNT Signaling