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Purpose
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Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.
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Immunogen
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Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).
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Isotype
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IgG
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Specificity
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Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins.
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Cross-Reactivity (Details)
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Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
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Characteristics
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Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
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Purification
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The product was purified from monospecific antiserum by Protein A affinity chromatography. Reactivity is directed against E.coli Host Cell Proteins.
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Labeling Ratio
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10-20 moles Biotin per mole of IgG
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