Combined Host Cell Proteins antibody (Biotin)

Details for Product No. ABIN6266438
Escherichia coli (E. coli)
ELISA, Western Blotting (WB)
Purpose Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.
Immunogen Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).
Isotype IgG
Specificity Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins.
Cross-Reactivity (Details) Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Characteristics Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
Purification The product was purified from monospecific antiserum by Protein A affinity chromatography. Reactivity is directed against E.coli Host Cell Proteins.
Labeling Ratio 10-20 moles Biotin per mole of IgG
Application Notes ELISA : 1:1000-1:2000
Western Blot : 1:500-1:1000

Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and is suitable for western blotting and ELISA.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 100 μL deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months