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Combined Host Cell Proteins antibody (Biotin)

Reactivity: E. coli ELISA, WB Host: Rabbit Polyclonal Biotin
Rockland
Catalog No. ABIN6266438
Supplier Product No.: 200-406-m61
  • Target
    Combined Host Cell Proteins
    Reactivity
    E. coli
    Host
    • 1
    Rabbit
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    Biotin
    Application
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB)
    Supplier Product No.
    200-406-m61
    Supplier
    Rockland
    Purpose
    Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.
    Characteristics
    Synonyms: rabbit anti-Host Cell Protein antibody Biotin conjugation, rabbit anti-HCP antibody, rabbit anti-E.Coli HCP antibody biotin conjugated, Anti-HCP biotin
    Purification
    Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins.
    Immunogen

    Immunogen: Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).

    Immunogen Type: Native Protein

    Isotype
    IgG
    Labeling Ratio
    10-20
  • Application Notes

    Application Note: Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and has been tested by western blot and is suitable for ELISA.

    Western Blot Dilution: 1:500-1:1000

    ELISA Dilution: 1:1000-1:2000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    Combined Host Cell Proteins
    Background
    Background: Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
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