Combined Host Cell Proteins antibody (Biotin)

Catalog No. ABIN6266438

Supplier Product No.: 200-406-m61

Product Details

Target: Combined Host Cell Proteins

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 200-406-m61

Supplier: Rockland

Purpose: Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.

Characteristics: Synonyms: rabbit anti-Host Cell Protein antibody Biotin conjugation, rabbit anti-HCP antibody, rabbit anti-E.Coli HCP antibody biotin conjugated, Anti-HCP biotin

Purification: Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins.

Immunogen:

Immunogen: Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Application Note: Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and has been tested by western blot and is suitable for ELISA.

Western Blot Dilution: 1:500-1:1000

ELISA Dilution: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Combined Host Cell Proteins

Background: Background: Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.