Topoisomerase II alpha antibody (AA 1352-1493)

Catalog No. ABIN6294306

This anti-Topoisomerase II alpha antibody is a Mouse Monoclonal antibody detecting Topoisomerase II alpha in WB, IHC, IF and FACS. Suitable for Human.

Quick Overview for Topoisomerase II alpha antibody (AA 1352-1493) (ABIN6294306)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1352-1493

Purpose: Mouse anti-Human TOP2A Antibody / Topoisomerase II alpha [Sodium Azide Free]

Specificity: Nuclear

Purification: Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.

Immunogen: Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10^6 cells in 0.1ml
IF: 1-2 μg/mL
Western blot: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: DNA topoisomerase 2-alpha

Background:

Target Description: This mAb recognizes an ~170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Gene Symbol: TOP2A///TOP2

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases