CD3 epsilon antibody (Cytoplasmic Domain)

Catalog No. ABIN6294325

This Mouse Monoclonal antibody specifically detects CD3 epsilon in FACS, WB, IHC and IF. It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow, Horse, Rabbit and Guinea Pig.

Quick Overview for CD3 epsilon antibody (Cytoplasmic Domain) (ABIN6294325)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 156-168, Cytoplasmic Domain

Purpose: Mouse anti-Human/Mouse/Rat/Rabbit/Guinea pig/Pig/Horse/Cow CD3e Antibody [Sodium Azide Free]

Specificity: Cell surface and cytoplasmic

Purification: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Immunogen: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Application Details

Application Notes: WB: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 min at RT
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: T-cell surface glycoprotein CD3 epsilon chain

Background:

Target Description: Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Gene Symbol: CD3E///T3E

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway