Villin 1 antibody (AA 179-311)

Catalog No. ABIN6294342

This anti-Villin 1 antibody is a Mouse Monoclonal antibody detecting Villin 1 in IHC, WB, IF and FACS. Suitable for Human.

Quick Overview for Villin 1 antibody (AA 179-311) (ABIN6294342)

Target: Villin 1 (VIL1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Villin 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Villin 1 Antibody

Binding Specificity: AA 179-311

Purpose: Mouse anti-Human Villin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic and cell surface

Purification: Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.

Immunogen: Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10^6 cells in 0.1ml
IF: 1-2 μg/mL
Western blot: 1-2 μg/mL
IHC (FFPE): 0.25-0.5 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: Villin-1

Background:

Target Description: Recognizes a protein of ~93 kDa which is identified as Villin (VIL1). It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Gene Symbol: VIL1///VIL

Gene ID: 7429

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization