Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2) (AA 280-420) antibody

Catalog No. ABIN6294441

The Mouse Monoclonal anti-Isocitrate Dehydrogenase [NADP],cytoplasmic antibody (ABIN6294441) specifically detects Isocitrate Dehydrogenase [NADP],cytoplasmic in IHC. The antibody is reactive with Human samples.

Quick Overview for Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2) (AA 280-420) antibody (ABIN6294441)

Target: Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 280-420

Purpose: Mouse anti-Human IDH1 Antibody [IHC only]

Specificity: Cytoplasmic & nuclear

Purification: A recombinant fragment (119 amino acid residues around aa 280-420) from the human protein was used as the immunogen for the IDH1 antibody.

Immunogen: A recombinant fragment (119 amino acid residues around aa 280-420) from the human protein was used as the immunogen for the IDH1 antibody.

Application Details

Application Notes: Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, For IHC use only

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details

Target: Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2)

Alternative Name: Isocitrate dehydrogenase cytoplasmic

Background:

Target Description: It recognizes a 45 kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming a-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of a-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.

Gene Symbol: IDH1///PICD

Gene ID: 3417

UniProt: O75874