TGFA antibody (AA 34-43)

Catalog No. ABIN6294480

This anti-TGFA antibody is a Mouse Monoclonal antibody detecting TGFA in IHC, IF and FACS. Suitable for Human, Rabbit and Zebrafish (Danio rerio).

Quick Overview for TGFA antibody (AA 34-43) (ABIN6294480)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human, Rabbit, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TGFA Antibody

Binding Specificity: AA 34-43

Purpose: Mouse anti-Human/Rabbit/Zebrafish TGF alpha Antibody [Sodium Azide Free]

Specificity: Cytoplasmic and secreted

Purification: A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.

Immunogen: A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (Formalin-paraffin): 1-2 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: Protransforming growth factor alpha

Background:

Target Description: TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

Gene Symbol: TGFA

Gene ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway