ENO2/NSE antibody (AA 416-433)

Catalog No. ABIN6294529

This Mouse Monoclonal antibody specifically detects ENO2/NSE in IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ENO2/NSE antibody (AA 416-433) (ABIN6294529)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-ENO2/NSE Antibody

Binding Specificity: AA 416-433

Purpose: Mouse anti-Human/Mouse/Rat NSE Antibody / Neuron Specific Enolase [IHC only]

Specificity: Cytoplasmic

Purification: Amino acids 416-433 of human NSE were used as the immunogen for this Neuron Specific Enolase antibody.

Immunogen: Amino acids 416-433 of human NSE were used as the immunogen for this Neuron Specific Enolase antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, For IHC use only

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: Gamma-enolase

Background:

Target Description: This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Gene Symbol: ENO2

Gene ID: 2026

UniProt: P09104