BRCA1 antibody (AA 445-620)

Catalog No. ABIN6294532

This Mouse Monoclonal antibody specifically detects BRCA1 in ELISA, IF, FACS and Coat. It exhibits reactivity toward Human.

Quick Overview for BRCA1 antibody (AA 445-620) (ABIN6294532)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Product Details anti-BRCA1 Antibody

Binding Specificity: AA 445-620

Purpose: Mouse anti-Human BRCA1 Antibody (Breast Marker) [Sodium Azide Free]

Specificity: Nuclear, cytoplasmic

Purification: Amino acids 445-620 were used as the immunogen for the BRCA1 antibody.

Immunogen: Amino acids 445-620 were used as the immunogen for the BRCA1 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL,ELISA: order the BSA free formulation for coating

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: Breast cancer type 1 susceptibility protein

Background:

Target Description: BRCA1 (Breast cancer type 1 susceptibility protein) is a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Gene Symbol: BRCA1///RNF53

Gene ID: 672

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus