KRT14 antibody (C-Term)

Catalog No. ABIN6294626

The Mouse Monoclonal anti-KRT14 antibody has been validated for IHC, IF and FACS. It is suitable to detect KRT14 in samples from Human, Rat and Mouse.

Quick Overview for KRT14 antibody (C-Term) (ABIN6294626)

Target: KRT14 (Keratin 14 (KRT14))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-KRT14 Antibody

Binding Specificity: C-Term

Purpose: Mouse anti-Human/Mouse/Rat Cytokeratin 14 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A 15 amino acid peptide from the C-terminus of human keratin 14 was used as the immunogen for this anti-Cytokeratin 14 antibody.

Immunogen: A 15 amino acid peptide from the C-terminus of human keratin 14 was used as the immunogen for this anti-Cytokeratin 14 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Keratin, type I cytoskeletal 14

Background:

Target Description: Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Gene Symbol: KRT14

Gene ID: 3861

UniProt: P02533