TGFA antibody (C-Term)

Catalog No. ABIN6294629

The Mouse Monoclonal anti-TGFA antibody is suitable to detect TGFA in samples from Human, Rat, Mouse and Cow. It has been validated for IHC, WB, IF and FACS.

Quick Overview for TGFA antibody (C-Term) (ABIN6294629)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TGFA Antibody

Binding Specificity: C-Term

Purpose: Mouse anti-Human/Cow/Rat/Mouse TGF alpha Antibody [Sodium Azide Free]

Specificity: Cytoplasmic and secreted

Purification: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.

Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
FACS:0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: Protransforming growth factor alpha

Background:

Target Description: TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

Gene Symbol: TGFA

Gene ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway