KRT6A antibody (C-Term)

Catalog No. ABIN6294644

The Mouse Monoclonal anti-KRT6A antibody has been validated for IHC, FACS and IF. It is suitable to detect KRT6A in samples from Human and Mouse.

Quick Overview for KRT6A antibody (C-Term) (ABIN6294644)

Target: KRT6A (Keratin 6A (KRT6A))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT6A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-KRT6A Antibody

Binding Specificity: C-Term

Purpose: Mouse anti-Human/Mouse Cytokeratin 6 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from the C-terminus of human Cytokeratin 6 was used as the immunogen for this antibody.

Immunogen: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from the C-terminus of human Cytokeratin 6 was used as the immunogen for this antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for KRT6A

Target: KRT6A (Keratin 6A (KRT6A))

Alternative Name: Keratin, type II cytoskeletal 6A

Background:

Target Description: This antibody recognizes a protein of 56 kDa, identified as cytokeratin 6 (CK6) or Keratin 6. In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Cytokeratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express Cytokeratin 6 and 16. Keratin 6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of Cytokeratin 6 is particularly associated with differentiation.

Gene Symbol: KRT6A///K6A///KRT6D

Gene ID: 3853

UniProt: P02538