Chromogranin A antibody

Catalog No. ABIN6295897

This Mouse Monoclonal antibody specifically detects Chromogranin A in IHC, FACS and IF. It exhibits reactivity toward Human, Rat, Mouse, Monkey and Pig.

Quick Overview for Chromogranin A antibody (ABIN6295897)

Target: Chromogranin A (CHGA)

Reactivity: Human, Rat, Mouse, Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Chromogranin A Antibody

Purpose: Mouse anti-Human/Monkey/Pig/Mouse/Rat Chromogranin A Antibody Cocktail [Sodium Azide Free]

Specificity: Finely granular cytoplasmic

Purification: Human pheochromocytoma cells (LK2H10 + PHE5) and recombinant human Chromogranin A protein (CGA414) were used as the immunogen for this antibody.

Immunogen: Human pheochromocytoma cells (LK2H10 + PHE5) and recombinant human Chromogranin A protein (CGA414) were used as the immunogen for this antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: Chromogranin-A , AL-11, GV-19, GR-44, ER-37, GE-25, Serpinin-RRG, Serpinin, p-Glu serpinin precursor]

Background:

Target Description: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Gene Symbol: CHGA

Gene ID: 1113

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling