Cytokeratin 5 antibody

Catalog No. ABIN6295942

This Mouse Monoclonal antibody specifically detects Cytokeratin 5 in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Mouse, Rat, Cow, Pig, Dog, Hamster, Sheep and Ferret.

Quick Overview for Cytokeratin 5 antibody (ABIN6295942)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Hamster, Sheep, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Cytokeratin 5 Antibody

Purpose: Mouse anti-Human/Pig/Mouse/Rat/Hamster/Cow/Dog/Sheep/Ferret Cytokeratin 5 + 8 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5 + 8 antibody.

Immunogen: A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5 + 8 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Keratin, type II cytoskeletal 5

Background:

Target Description: It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Gene Symbol: KRT5///KRT8///CYK8

UniProt: P13647, P05787