Nkx2-2 antibody

Catalog No. ABIN6296003

The Rabbit Polyclonal anti-Nkx2-2 antibody has been validated for IF, IHC and FACS. It is suitable to detect Nkx2-2 in samples from Human.

Quick Overview for Nkx2-2 antibody (ABIN6296003)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Nkx2-2 Antibody

Purpose: Rabbit anti-Human NKX2.2 Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: A human partial recombinant protein was used as the immunogen for this NKX2.2 antibody.

Immunogen: A human partial recombinant protein was used as the immunogen for this NKX2.2 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10e6 cells in 0.1ml
IF: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 minutes at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: Homeobox protein Nkx-2.2

Background:

Target Description: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.

Gene Symbol: NKX2-2///NKX2.2///NKX2B

Gene ID: 4821

UniProt: O95096

Pathways: Dopaminergic Neurogenesis