CD31 antibody

Catalog No. ABIN6296034

The Mouse Monoclonal anti-CD31 antibody (ABIN6296034) specifically detects CD31 in FACS, WB, IHC and IF. The antibody is reactive with Human, Rabbit and Cynomolgus samples.

Quick Overview for CD31 antibody (ABIN6296034)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human, Rabbit, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD31 Antibody

Purpose: Mouse anti-Human/Cynomolgus Monkey/Rabbit CD31 Antibody [Sodium Azide Free]

Specificity: Cell surface and cytoplasm of endothelial cells

Purification: A membrane preparation of a spleen from a patient with hairy cell leukemia was used as the immunogen for this CD31 antibody.

Immunogen: A membrane preparation of a spleen from a patient with hairy cell leukemia was used as the immunogen for this CD31 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: Platelet endothelial cell adhesion molecule

Background:

Target Description: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Gene Symbol: PECAM1

Gene ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization