CD5 antibody

Catalog No. ABIN6296063

The Mouse Monoclonal anti-CD5 antibody is suitable to detect CD5 in samples from Human, Rat, Cow, Rabbit, Horse, Pig, Monkey, Chicken and Guinea Pig. It has been validated for FACS, WB, IHC and IF.

Quick Overview for CD5 antibody (ABIN6296063)

Target: CD5

Reactivity: Human, Rat, Cow, Rabbit, Horse, Pig, Monkey, Chicken, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD5 Antibody

Purpose: Mouse anti-Human/Monkey/Horse/Pig/Rabbit/Cow/Rat/Guinea Pig/Chicken CD5 Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: A peptide from the intracellular region of the human protein was used as the immunogen for this CD5 antibody.

Immunogen: A peptide from the intracellular region of the human protein was used as the immunogen for this CD5 antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD5

Target: CD5

Alternative Name: T-cell surface glycoprotein CD5

Background:

Target Description: Recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Gene Symbol: CD5///LEU1

Gene ID: 921

UniProt: P06127