CD3 epsilon antibody

Catalog No. ABIN6296157

The Rabbit Polyclonal anti-CD3 epsilon antibody (ABIN6296157) specifically detects CD3 epsilon in FACS, WB and IP. The antibody is reactive with Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig, Chicken, Dog, Sheep, Hamster, Cat, Cryptomys amatus (Mole rat), Elephant, Frog, Opossum and Primate samples.

Quick Overview for CD3 epsilon antibody (ABIN6296157)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig, Chicken, Dog, Sheep, Hamster, Cat, Cryptomys amatus (Mole rat), Elephant, Frog, Opossum, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-CD3 epsilon Antibody

Purpose: Rabbit anti-Human/Mouse/Rat/Primate/Guinea Pig/Hamster/Horse/Mole Rat/Pig/Rabbit/Sheep/Cat/Chicken/Cow/Dog/Frog/Opossum/Elephant CD3e Antibody [Sodium Azide Free]

Specificity: Cell surface and cytoplasmic

Purification: Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.

Immunogen: Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.

Application Details

Application Notes: FACS: use the CD3e antibody at 0.5-1 μg/million cells
WB: 0.5-1.0 μg/mL
IP: 1-2 μg/500 μg protein lysate,

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: T-cell surface glycoprotein CD3 epsilon chain

Background:

Target Description: This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.

Gene Symbol: CD3E///T3E

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway